ABSTRACT
Objective To establish a QAMS method for content determination of six compositions (chlorogenic acid, caffeic acid, cryptochlorogenin acid, isochlorogenic acid A, isochlorogenic acid C and loganin) from Lonicerae Japonicae Caulis; To verify the feasibility and applicability of this method in quality control of Lonicerae Japonicae Caulis. Methods Chlorogenic acid was set as internal reference substance. The HPLC analysis was performed on a Waters Symmetry C18 column (4.6 mm × 250 mm, 5 μm) with a mobile phase consisted of acetonitrile and 0.4% phosphoric acid solution in gradient elution manner at a flow rate of 1 mL/min. The column temperature was maintained at 35 ℃, and the detection wavelength was set at 327 nm for chlorogenic acid, caffeic acid, cryptochlorogenin acid, isochlorogenic acid A, isochlorogenic acid C and 236 nm for loganin. Results The relative correction factors of caffeic acid, cryptochlorogenin acid, isochlorogenic acid A, isochlorogenic acid C and loganin were established; there was no obvious difference between calculated value of QAMS and measured value of external standard method. Conclusion The quality control mode of QAMS can be used for multi-index synchronization quality evaluation of the six compositions from Lonicerae Japonicae Caulis.
ABSTRACT
OBJECTIVE: To establish the HPLC methods for determining the contents of the active ingredients and fingerprint chromatogram of Scutellaria barbata formula granules. METHOD: The HPLC analysis was carried out on a Wondasil C18 column (4.6 mm×250 mm, 5μm) with mobile phase consisting of methanol-acetonitrile-0.1% phosphoric acid (12.5:15:72.5) at the flow rate of 1.0 mL·min-1. The column temperature was maintained at 30t. The detection wavelength was set at 335 nm to determine the contents of scutellarin and scutellarein and 320 nm to establish the fingerprint chromatogram of Scutellaria barbata formula granules, medical materials, and aqueous decoction. RESULTS: The linear ranges of scutellarin and scutellarein were 0.074 0-0.518 0 μg and 0.051 6-0.3612 μg, respectively. The average recoveries were 103.18% and 99.99%, respectively. Nine peaks in the fingerprint chromatogram of formula granules could be tracked in the aqueous decoction, and eight peaks in the fingerprint chromatogram could be tracked in the medical materials. CONCLUSION: The methods can provide more information for the quality control of Scutellaria barbata formula granules.